Identification of the Unknown Bacteria

The Identification of the Unknown # Rachel Taylor For MCB 2010L-611 Dr. Digirolamo Procedures, Procedures, & Results: 1. Chose a designated # for an cabalistic bacterium that will be identified through a straight publication of prove and procedures as follows-#718. a. Each day a wise LB slant will be inoculated with unknown quantity bacterium and incubated for 24Hrs discarding previous organ pipe/tubes. 2. A simple dishonor of unknown bacteria was performed to determine that it is a Gram damaging nuance and morphological characteristics are of short/medium superstar and opposite rods. 3. Inoculate two LB agars, using aseptic proficiency for all inoculations, with one labeled 25 degrees and the former(a) at 37 degrees. Incubated for 24Hrs, recovered, and corresponded for optimal temperature for growth of unknown; which was the LB agar at 37 degrees. a. Catalase turn up performed after 24Hr incubation, test by mething a small a mount of bacteria from LB agar of 37 degrees, transferred to a clean scrap slide. Add one to two drops of H2O2 and observe for bubbling. Result was ban for catalase, bubbles were not present. 4. Next, the biochemical reactions tests are performed.

Inoculated three Durham tubes of phenol red broth with unknown bacteria, each containing a carbohydrate- sucrose, glucose, and lactose as a substrate. Incubated at 37 degrees for 24 hrs. colorimetric changes are wasting diseased to observe fermentation and bubbles tube to check for gas. Results as follows: Glucose was sultry (+), gas (+)-Bubbles present in tube/ xanthous pigment =Indicates total utilization of carbohydrates; sucro se was acid (+), gas (-)-No bubbles present/! Magenta pigment=Indicates incomplete use of carbohydrates; Lactose was acid (+), gas (+)-Bubbles present/Yellow pigment=Indicates total utilization of carbohydrates. 5. A gelatin dig is performed of unknown bacteria and incubated at 25 degrees for 24Hrs, the result was blackball indicating no liquefactionno gelatinase, still solid appearance of gelatin....If you neediness to need a full essay, order it on our website: OrderEssay.net

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